Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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Origins of cultivated potatoes and species relationships. In this paper we describe the fusion of leaf protoplasts of S.

The enzyme treatment was carried out overnight with gentle agitation. Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum protoplawtos.

Fusion de Protoplastos by Arely Gutierrez on Prezi

Somatoclonal variation in potato: Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange. Plant Cell Rep 3: Somatic hybrids were confirmed morphologically under greenhouse conditions, when peotoplastos characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed.

Chromosome observations indicated that all hybrids were aneuploids. Protoplasos fusion, protoplashos and regeneration Prior to fusion, protoplasts from S. We describe here fusion between phospholipid vesicles liposomes and protoplasts to the fungus phycomyces blakesleeanus.

These results showed that all hybrids were aneuploids Fig. Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape. Plant material and protoplast isolation. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. None of the plants had a similar banding pattern in relation to each one of the parents.

The RAPD analysis was carried out for all regenerated plants derived from calli of the combination S. Protoplast fusion, culture and regeneration.


Cultivo y fusion de protoplastos

Then the suspension was centrifugated at rpm during protoplasstos minutes. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: Scribd is the worlds largest social reading and publishing site.

Universidad Nacional Agraria La Molina. Non-transgenic applications of plant tissue culture in potato. Interspecific somatic hybridization between Solanum tuberosum L. Prior to fusion, protoplasts from S. Regeneration in Gewebekulturen einiger Dihaploider Solanum tuberosum Z. Regenerants from the fusion experiments were fusi in vitro using the Murashige and Skoog medium.

Shoot regeneration started twelve weeks after calli were transferred to MS medium. The morphology of fusion-derived regenerants was intermediate between the donor parents. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.

Gazala nasim were incubated in different concentrations of mannitol and combinations of enzyme solutions. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established. Somatic potato hybrids have been produced by fusion of diploid wild species with tetraploid S.

These bands also appeared in the fusion regenerants Figs.

Cultivo y fusion de protoplastos [1990]

These samples were obtained from rooted plants maintained in a greenhouse. Production of somatic hybrids by electrofusion in Solanum. The other parent, the wild species S. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur.

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The latter has a natural resistance to late blight, Phytophthora infestans Mont. Molecular genetic characterization of plant somatic tusin. Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. For this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications.

Fusion de protoplastos pdf

The production of somatic hybrid plants fusih a chromosome number deviating from the expected is well documented in many fusion combinations where potato protoplasts have been used as one or both fusion parents Austin et al. Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents. Introduction Potato, Solanum tuberosum L. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis.

None of the regenerants had 72 chromosomes, which results from the protoplasts fusion of a tetraploid with a diploid genome. The authors would like to thank the International Protopkastos Center for financial support and to Martha Williams de Castro who revised the English version of this article. This value is different from that obtained by Mattheij et al.

This technique is a tool for somatic hybrid characterization Baird et al. Protoplasts were precipitated by centrifugation at rpm during 10 minutes. Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but little aneuploidy; prtoplastos the other hand, regenerated plants from tetraploid genotypes produce a wide range of aneuploidy Kumar, After seven days, the culture medium was diluted 1: